Hieff qpcr sybr
Web6 de abr. de 2024 · The cDNA was then used for the RT-qPCR analysis using specific primers (Table S1) and Hieff® qPCR SYBR Green Master Mix (YE SEN, China). RT-qPCR was performed using the CFX96 Touch™ real-time PCR Detection System (BioRad, USA). β-actin was used as an internal reference gene. The qPCR program was as follows: 5 … Web9 de out. de 2024 · Hieff UNICON® Universal Blue qPCR SYBR Green Master Mix用Universal ROX替代了传统ROX,弥补不同仪器平台的光程差,实现了适合所有qPCR仪 …
Hieff qpcr sybr
Did you know?
Web2×Hieff™ PCR Master Mix contains Hieff™ Taq DNA Polymerase (Cat#10101), dNTPs, and other PCR-required components. The Master Mix is stable for 3 months at 4°C with our customized stabilizers. The pre-mix solution is optimized for conventional PCR and ready to use by adding DNA template and primers. The PCR products can be loaded directly ... Web28 de set. de 2024 · The qPCR reaction was performed using a QuantStudio 5 real-time fluorescence quantitative PCR system (Applied Biosystem, CA, USA) and Hieff qPCR SYBR Green Master Mix (Yeasen Biotech, Shanghai ...
WebHieff® qPCR SYBR Green Master Mix(No Rox)是2×实时定量PCR扩增的预混合溶液。 Mix中含有热启动Hieff ® DNA Polymerase、SYBR Green I、dNTPs、Mg 2+ 。 使用时,仅需在扩增体系中加入模板和引物即可进行实时荧光定量PCR,大大简化操作过程,降低污染 … WebiQ™ SYBR ® Green supermix is a 2x concentrated, ready-to-use reaction master mix optimized for dye-based quantitative PCR (qPCR). It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl 2, SYBR ® Green I dye, enhancers, stabilizers, and fluorescein. Instrument Compatibility
http://www.bio-toyobo.cn/pdf/QPK-201CH.pdf Web16 de mai. de 2024 · The RT-qPCR reactions were set up using cDNA, forward/reverse primer, and Hieff™ qPCR SYBR® Green Master Mix (Yeasen, Shanghai, China), according to manufacturer’s instructions. The reactions were carried out in 96-well PCR reaction plates in a LightCycler 480 system (Roche Molecular Biochemicals, Mannheim, Germany). 2.5. …
Web1. 介绍在定量PCR(也称rt-PCR或qPCR)中,荧光信号是由荧光探针或荧光DNA结合染料(如SYBR Green)产生,其强度与PCR产物分子(扩增子)的数量成正比。每个循环 …
Web湖南医药学院第一附属医院关于其他互作相关试剂的网上超市采购项目合同履约验收公告 legendary buffalo wild west robloxWeb22 de fev. de 2024 · Gene expression was determined by Hieff qPCR SYBR Green Master Mix (no ROX) (#11201ES03, Yeasen), and gene expression was normalized to β-actin using the ΔΔCt method. legendary bronze tanning lotionWeb7 de set. de 2024 · The other components including 2 × Hieff ® qPCR SYBR Green Master Mix 5 μL, standard plasmid DNA template (1.0 × 10 3 copies/μL) 1 μL, and nuclease-free water were added to make a total volume of 10 μL. The amplification reaction was performed in the LightCycler ... legendary buck thermalWeb28 de out. de 2024 · RT-qPCR analysis. The expression of carrot carotenoid pathway genes under 15% PEG 6000 treatment was detected by RT-qPCR according to the instructions of Hieff qPCR SYBR Green Master Mix (Shanghai Yeason Biotechnology Co., Ltd.). DcActin1 gene was used as the reference gene (Xu et al. 2014). legendary buffy deck building gamehttp://img1.bioon.com/reagent/Show_product.asp?id=1179333 legendary buffalo rdr2Web2 de abr. de 2014 · Quantitative real-time PCR is PCR visualized in real time by the use of fluorescent or intercalating dyes, which are employed to measure gene expression or gene quantification including contiguous gene deletions or duplications. legendary bug type pokemonWebHieff UNICON ® Universal Blue qPCR SYBR Green Master Mix 实时荧光定量PCR扩增预混液. 11184ES08. 5× 1 mL. 1653.00. 663.00. Hieff UNICON ® Universal Blue qPCR … legendary bulletproof vest cyberpunk