Bcdat medium
WebFeb 8, 2024 · BCDAT medium was additionally supplemented with 1 mM ammonium tartrate 50. Plants were grown at 24 o C with a 16 h: 8 h, light (300mmol m-2 s-1): dark cycle. To induce sporophytes, tissues were grown for 3-6 months on peat plugs at 16°C with an 8 h: 16 h, light (300mmol m-2 s-1): dark cycle. Sporangia were sterilized in 20% … WebMay 20, 2016 · Chloronema-to-caulonema conversion takes place more rapidly on the BCD medium than on the BCDAT medium. 19. It is convenient to keep some BCD agar media in a 1.5 ml tube, since the tube can be heated in the heat block and the medium becomes molten quickly (100 °C, 2 min). 20. A thinner layer of the agar medium is better for clear …
Bcdat medium
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WebAfter bleomycin treatment, tissue was thoroughly rinsed in water, blotted on filter paper and either flash-frozen in liquid N 2 (repair t = 0) or left to recover on plates in liquid BCDAT medium ... Web, 0.17 mM KI) agar medium supplemented with 1mM CaCl 2 and 5mM ammonium tartrate (BCDAT medium), or as lawns of protonemal filaments by subculture of homoge-nized tissue on BCDAT agar medium overlaid with cellophane for the isolation of protoplasts. Bacterial strains and constructs Plasmid Tnk23 (Lucas etal., 1995) is a derivative of the …
WebAug 17, 2024 · The gametophores were then covered with solid BCDAT medium with 10 µg ml −1 PI without zeocin, and observation was resumed. Bright-field and PI fluorescence … WebMar 15, 2024 · Phenotypic analysis of WT and transgenic lines. (A) Cell lines were grown in BCDAT medium for 35 days and photographed using Miotic SMZ-171 microscope.(B) Cells grown in similar conditions as ...
WebFeb 16, 2007 · For immunofluorescence, tissue grown on BCDAT or BCD medium was harvested 10 and 15 d after inoculation. Immunofluorescence Microscopy. Tissue from P. patens cultured on BCDAT or BCD medium was prepared for immunofluorescent localization of mannan as described previously (McCartney et al., 2003). Briefly, tissue … WebFeb 12, 2024 · BCDAT medium : Add 10 mL of stock B, 10 mL of stock C, 10 mL of stock D, 10 mL of 500 mM ammonium tartrate, 1 mL of alternative TES, and 20 mL of 50 mM CaCl 2 to 900 mL of water, and fill to 1000 mL with water. For solid medium, add 8 g of agar per 1000 mL of medium. 3. BCDATG medium : BCDAT medium supplemented with 5 g/L …
WebMay 25, 2024 · BCDAT medium: BCD medium supplemented with 5 mM diammonium (+)-tartrate. 3. BCDATG medium: BCDAT medium supplemented with 0.5% glucose. 2.4 Solutions and Media for Transformation 1. 8% Mannitol: Dissolve 8 g D-mannitol in 100 ml H 2 O and sterilize by autoclaving. Store at room temperature. 2.
WebJan 26, 2024 · After regeneration on PRMB medium containing 3 µM PABA, four-day-old transformed protoplasts were selected on BCDAT medium containing 3 µM PABA, 100 mg/l gentamicin, and 20 mg/l G418 for one week. Transformants were picked to BCDAT+PABA medium, then 10 days later a small clump of each was transferred to … don\u0027t starve together cookbookWebJun 1, 2016 · The body plan of the early-diverged moss Physcomitrella patens (P. patens) is relatively simple. The vegetative gametophyte is composed of only a few cell types and developmental stages. Auxin was … city of houston grass clippings pickupWebFeb 6, 2024 · Protonemata were dispersed in liquid BCDAT medium containing bleomycin or MMS and treated for 1 h. After treatment and washing recovered protonemata were inoculated as eight explants per quadrant of a Petri dish with drug-free BCDAT agar without cellophane overlay and left to grow. city of houston great west retirementWebJul 22, 2024 · Survivors were transferred to plates containing BCDAT medium, and then the plates were vertically oriented in darkness for phenotypic screening (Fig. 1a ). Several mutants whose protonemata grew... city of houston.gov jobsWebDec 1, 2024 · All plant materials were cultured on BCDAT medium with 1 mM CaCl 2 at 25℃ under a 16 h/8 h (light/dark) photoperiod with the light intensity of 60−80 μmol … city of houston golfWebOn minimal BCD medium, protonemal growth was strongly reduced and eventually ceased after a month (Figure 2A, C and G). At this stage, colonies comprised both chloronemal and caulonemal cells and... don\u0027t starve together co op local pcWebJan 28, 2024 · GFP-mTalin transformants for cdka-dko deficiency were selected on BCDAT medium containing hygromycin (30 mg/liter) or zeocin (100 mg/liter) and further confirmed by polarotropism defects under polarized light. To observe cp-actin, a confocal laser scanning microscope was used (Zeiss LSM 510). The objective lens was a Plan … don\u0027t starve together cook